Report Number: 12819
Permit Number: CRLA1997ASPN
Date Received: Jan 01, 1998
Reporting Year:
1997
Principal Investigator:
Shannon Datwyler, Dept of Plant Biology,
Ohio State University, Columbus, OH
Park-assigned Study Id. #:
CRLA1997ASPN
Permit Expiration
Date:
Jan 01, 1998
Permit Start Date:
Jan 01, 1997
Study Starting
Date:
Jan 01, 1997
Study Ending Date:
Jan 01, 1997
Study
Status:
Completed
Activity Type:
Research
Subject/Discipline:
Plant Communities (Vegetation)
Objectives:
Population structure and haabitat
preferences in hybrid swarms.; a. Elucidate
the extent of hybridization on Wizard Island
and determine the genealogical relationships
of the plants in the population.; b. Habitat
specialization. Determine whether hybrids
and parental taxa occur in similar or
distinct habitats.;Assess Evolutionary
implications of gene flow.; a. Patterns of
gene flow. Determine patterns of
directionality and distance of pollen
movement on Wizard Island.; b.
Homogenization of genotypes/gene swamping.
Assess whether patterns of gene flow suggest
potential for homogenization of geotypes or
gene swamping in an isolated population.
Findings and Status:
Leaf material and one or two stems were
collected from five populations in CRLA NP.
Two Population each of "pure"P.davidsonii
and "pure" P.rupicola were collected from
rim populations. From each pop., twelve
specimens were measured and leaf samples
were taken and one to five stems were
removed for voucher specimens. Voucher
specimens will be deposited at OSU
herbarium. On Wizard Island, 161 individual
plants were tagged with aluminum tags,
mapped, measured and leaves were removed for
DNA extractions and stems were removed for
voucher specimens.;The DNA extractions have
been completed for all of the plants sampled
(209 total sammples) and parental markers
are being established for P.davidsonii and
P.rupicola using inter-simple sequence
repeat markers (ISSRs). Morphological data
has been entered and is being analyzed in
order to establish morphological markers
between parental taxa. Morphological markers
as well as molecular markers will be used in
order to determine the degree of hybridity
within the Wizard Island hybrid swarm.
For
this study, were one or more specimens collected and
removed from the park but not destroyed during
analyses?
No
Funding provided
this reporting year by NPS:
0
Funding provided
this reporting year by other sources:
0
Full name of
college or university: n/a
Annual funding
provided by NPS to university or college this
reporting year:
0
